Elisa development kit contains the key components required for the quantitative measurement of natural andor recombinant human ifn. Ifn gamma is a proinflammatory cytokine secreted by t and nk cells in response to viral infection and other inflammatory conditions. Detailed procedures for enzymelinked immunospot elispot and introduction to how elispot works. Human ifn gamma elisa kit ab174443 is a singlewash 90 min sandwich elisa designed for the quantitative measurement of ifngamma protein in human cell culture supernatant, plasma. Verikine human ifn beta elisa kit pbl assay science. Mouse interferon alpha 2 elisa kit ab211648 is a singlewash 90 min sandwich elisa designed for the quantitative measurement of interferon alpha 2 protein in cell culture extracts, cell culture supernatant, plasma, serum, and tissue extracts. Elisa enzymelinked immunosorbent assay kit is an in vitro enzymelinked immunosorbent assay for the quantitative measurement of rat ifn. Intended use quantiferontb gold qft is an in vitro diagnostic test using a peptide cocktail simulating esat6, cfp10, and tb7.
Elisa technical guide and protocols thermo fisher scientific. Opened reconstituted reagents standard sample diluent may be stored for up to 1 month at. Refer to the lotspecific c of a for amount supplied. The following is a protocol that was developed for the tgf. Using the elisa protocol, the recommended microplates, reagents and solutions, the components supplied in this kit are sufficient to assay human ifn. Human interferon beta ifn beta elisa kit from elisa genie is a precoated immunoassay with a sensitivity of 18. Human interferon alpha 1 elisa kit ab2479 is a singlewash 90 min sandwich elisa designed for the quantitative measurement of interferon alpha 1 protein in cell culture extracts, cell culture supernatant, serum, tissue extracts, cit plasma, edta plasma, and hep plasma. A monoclonal capture antibody specific for human ifn. The human ifn alpha elisa is for research use only. Cytokinespecific monoclonal capture antibodies are immobilized on an ethanoltreated pvdf membrane plate. The ifn human elisa kit is designed to detect and quantify the level of human interferon alpha ifn in media using 96well plates and a microplate reader. Sufficient materials are provided to yield approximately 20 plates of 96wells if the. The verikinetm mouse interferon beta elisa kit has been developed to quantify levels of ifn.
Originally described by engvall and perlmann 1971, the method enables analysis of protein samples immobilized in microplate wells using specific antibodies. Production find, read and cite all the research you need on researchgate. Elisa max deluxe elisa development systems engineered for sensitive, precise and reliable serum and plasma cytokine quantitationbiolegends elisa max sets. The assay is based on the international reference standard for human interferon alpha hu ifn. Includes elisa plates and reagents for 480 tests 5 x 96 wells our research use assay uses two different mouse anti bovine interferon gamma ifn. Ifn gamma human elisa kit, high sensitivity invitrogen. The assay will exclusively recognize both natural and recombinant hu hu ifn principle of the method. Dilute the antigen to 12 ugml in coating solution 2. It uses our proprietary simplestep elisa technology. Bowdish lab, mcmaster university hamilton, on, canada. Interferon alpha human elisa kit thermo fisher scientific. This mouse ifn gamma uncoated elisa contains the necessary reagents, standards, buffers and diluents for performing quantitative enzymelinked immunosorbent assays elisa. A userfriendly, highly sensitive assay to detect the ifn.
Human ifn alphabeta r2 elisa kit ifnar2 ab264610 abcam. The assay is based on the international reference standard for human interferon beta hu ifn. Easytorun sandwich elisa has a total assay incubation time is only 2. Human ifn gamma high sensitivity elisa kit ab236895 is a singlewash 90 min sandwich elisa designed for the quantitative measurement of ifn gamma protein in cit plasma, edta plasma, saliva, serum, and cell culture supernatant. Elisa kit for cell and tissue lysates sigmaaldrich. In our plate coating guide you can find detailed information on how to perform. Elisa is an enzymelinked immunosorbent assay for the quantitative detection of human ifn the human ifn. Elisa for natural or recombinant human ifng in serum, plasma, and cell culture supernatants. Quantitate human ifn gamma with 470 pgml sensitivity. Results obtained for naturally occurring human ifn gamma samples showed linear curves that were parallel to the standard. Human ifn gamma elisa kit ab174443 is a singlewash 90 min sandwich elisa designed for the quantitative measurement of ifn gamma protein in human cell culture supernatant, plasma and serum samples.
A targetspecific antibody has been precoated in the wells of the supplied microplate. Use clean, dedicated reagent trays for dispensing the washing liquid. This duoset elisa development kit contains the basic components required for the development of sandwich elisas to measure natural and recombinant mouse ifn gamma. The opteia set for mouse interferon g ifn g contains the components necessary to develop enzymelinked immunosorbent assays elisa for natural or recombinant mouse ifn g in serum, plasma, and cell culture supernatants. The human interferon beta ifn beta elisa kit accurately measures natural human interferon beta.
Peg interferon alpha2a pegasys 45 microgram subcutaneously weekly peg interferon alpha2b is not recommended due to lack of experience. The enzyme linked immunosorbent assay elisa is a powerful method for detecting and quantifying a specific protein in a complex mixture. Ifna interferonalpha elisa kits the elisa enzymelinked immunosorbent assay is a widely used application for detecting and quantifying proteins and antigens from various samples. Uncoated elisa the following protocol is a general guideline for the uncoated sets materials provided please refer to the certificate. Results obtained for naturally occurring human ifn gamma samples showed.
Interferongamma elisa protocol coat plate with 50ul of a 2. Mouse ifn no crossreactivity detected with rat ifn. Add 100 ul of diluted antigen to appropriate wells. Sets provide all the timesavings, convenience and performance of a highend, commercial elisa kit for a fraction of the price. Type i interferons induced by radiation therapy mediate. Ifn gamma plays a major role in innate and adaptive immunity by promoting t cell differentiation into th1 and the development of treg, as well as by inhibiting t cell differentiation into th17. Human ifn alpha interferon alpha elisa kit elisa genie.
Mix all reagents of the human interferon gamma ifn. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Ifn alpha interferon alpha is a type i interferon belonging to a family of structurally related, speciesspecific proteins exclusive to vertebrates. Elisa with streptavidin conjugated to horseradish peroxidase hrp. The enzymelinked immunospot elispot assay is a sensitive method for quantification of the number of cytokine secreting cells. The interaction of ifn alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. Activation of ifn gamma takes place through binding of ifn gamma receptor i and ii, and activating the jakstat pathway. The assay will exclusively recognize both natural and recombinant ms ifn principle of the method. Human interferon beta ifn beta elisa kit elisa genie. The data collected includes not only links to publications in pubmed, but also provides information about sample types, species, and experimental conditions. Elisa this antibody is immobilized to a microtiter plate. Dilute in pbs without carrier protein to the working. Human ifn alpha platinum elisa thermo fisher scientific. The enzymelinked immunosorbent assay elisa is a frequently used application which determines the concentration of an analyte in solution.
Quantitate human ifn gamma with 2 pgml sensitivity. Sets, in two appealing formats, address the need for. Sets, in two appealing formats, address the need for sensitivity. Mouse interferon gamma ifn elisa assay kit 410 catalog number. Elisa kit this product is suitable for in vitro quantitative detection of mouse serum, plasma or cell culture supernatant and organizations in. Use clean, dedicated reagent trays for dispensing the washing liquid, conjugate and substrate reagent. Mouse ifn gamma uncoated elisa components of 10plates. Elisa max deluxe elisa development systems engineered for sensitive, precise and reliable serum and plasma cytokine quantitationbiolegends elisa max sets provide all the timesavings, convenience and performance of a highend, commercial elisa kit for a fraction of the price.
Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use. Human ifn alpha interferon alpha elisa kit from elisa genie is a precoated immunoassay with a sensitivity of 9. Ifn gamma does not show any homology with ifn alpha or. Interferons ifns activity has been discovered due to their antiviral effects. Invitrogen ifn gamma human uncoated elisa kit with plates. Elisa enzymelinked immunosorbent assay kit is an in vitro enzymelinked immunosorbent assay for the quantitative measurement of mouse ifn. Pdf on jan 1, 2017, michelle wykes and others published elispot assay to measure peptidespecific ifn. Mouse interferon alpha 2 elisa kit ab211648 is a singlewash 90 min sandwich elisa designed for the quantitative measurement of interferon alpha 2 protein in cell culture extracts, cell. Mbs2512904 96t48t24t this manual must be read attentively and completely before using this product. Ifn gmcsf the biomarker reference set for cancers in women brscw was provided by the national cancer institute on behalf of the early detection research network edrn.